Disposable series of laparoscopic puncture device. Laparoscopic puncture device for laparoscopic surgery puncture, the establishment of surgical instruments into the human body channel, suitable for minimally invasive surgery. The innovative double-layer sealing design has excellent dynamic and static air tightness. After the instrument with a diameter of 12mm is removed, the sealing time only needs 0.7S, which greatly ensures the life safety of patients. The design of silicone sealing rings with different wall thicknesses and micro-shapes, which has been repeatedly demonstrated, aims at allowing surgical instruments with different diameters to enter and exit quickly and smoothly, and the insertion and removal resistance can be 0.3-0.5N. The two-stage ring pattern design of the inverted ring on the puncture casing has small puncture resistance and large pull-out resistance, which is conducive to the fixation of the puncture device with the abdominal wall and reduces the damage to the tissue caused by the movement of the instrument during the operation. The ergonomic grip handle improves the comfort and stability of the doctor's hand during puncture.